Evidence for Prostanoid Biosynthesis as a Biochemical Feature of Certain Subclasses of Non-Small Cell Carcinomas of the Lung as Determined in Established Cell Lines Derived from Human Lung Tumors1

Detectable levels (>0.2 pinol/ID" cells) of one or more prostanoid species resultant to calcium ionophore A23187-induced biosynthesis from endogenous arachidonic acid were distributed in 28 cell lines derived from different histolÃ3gica!classes of lung tumors as follows: large cell undifferentiated carcinoma (3 of 3 cell lines); adenosquamous carcinoma (1 of 2 cell lines); squamous cell carcinoma (0 of 2 cell lines); adenocarcinoma (9 of 10 cell lines); bronchioloalveolar cell carcinoma (2 of 2 cell lines); and small cell carcinoma (1 of 9 cell lines). Using the mean levels of 9a,110-prostaglandin F2, prostaglandin !•':.,. prostaglandin D2, prostaglandin 1\ thromboxane B2and 6-keto-prostaglandin !•',, as an index of prostaglandin H (PGH) synthase activity, the distribution in cell lines representative of the different histolÃ3gica!classes of human lung tumors exhibiting PGH synthase activity exceeding mean values >2 pmol/106 cells was as follows: large cell undifferentiated carcinoma (3 of 3 cell lines), adenosquamous carcinoma (1 of 2 cell lines), adenocarcinoma (8 of 10) cell lines), bronchioloalveolar cell carcinoma (2 of 2 cell lines) and small cell carcinoma (0 of 9 cell lines). Three different prostanoid species accumulated to mean levels >2 pmol/106 cells. Prostaglandin K; levels exceeded 2 pmol/106 cells in 14 of the 16 cell lines in which this prostanoid accumulated to detectable levels. Cumulative levels of prostaglandin !•':., exceeded 2 pmol/106 cells in 9 of the 15 cell lines in which prostaglandin I1';,,reached detectable levels. Detectable levels of thromboxane B2 were observed in five cell lines with thromboxane B2accumulation exceeding 2 pmol/106 cells in two of the five cell lines. 9a,l l/?-prostaglandin F2 and 6-keto-prostaglandin 1'V,accumulated to detectable levels in the culture medium of one cell line, while prostaglandin I>:accumulation to detect able levels was observed in two cell lines. Stimulation of cultured human lung tumor cells exhibiting PGH synthase activity >2 pmol/106 cells in the presence of ID""'M exogenous arachidonic acid resulted in a 2to 4fold increase in the accumulation of individual prostanoids, while the inclusion of a 10~*M exogenous concentration of arachidonic acid failed to stimulate detectable prostanoid production in human lung tumor cells in which PGH synthase activity was not previously expressed. The predominance of PGH synthase activity in cell lines derived from human non-small cell carcinomas of the lung suggest that prostanoid biosynthesis may be characteristic of tumor cells comprising certain histolÃ3gica! subclasses of human non-small cell carcinomas of the lung, particularly adenocarcinoma, bronchioloalveolar cell carcinoma, large cell undiffer entiated carcinoma, and possibly adenosquamous carcinoma.